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Description
Fibrillarin Recombinant Rabbit mAb (S-1718-171)Product Specification Host Rabbit Antigen Fibrillarin Synonyms rRNA 2' O methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone glutamine methyltransferase; U6 snRNA 2' O methyltransferase fibrillarin; FIB1; FLRN; FBL Immunogen Synthetic Peptide Location Nucleus Accession P22087 Clone Number S 1718 171 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rt, Mk Positive Sample HEK 293, HeLa,
Product Specification
| Host | Rabbit |
| Antigen | Fibrillarin |
| Synonyms | rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase; U6 snRNA 2'-O-methyltransferase fibrillarin; FIB1; FLRN; FBL |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P22087 |
| Clone Number | S-1718-171 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, HeLa, HepG2, Molt-4, Jurkat, NIH/3T3, mouse liver, C6, rat testis, COS-7 |
| Predicted Reactivity | Dr, Xe, Fu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:50-1:100 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Fibrillarin is an evolutionarily conserved, 34-kDa nucleolar methyltransferase that is essential for 2'-O-methylation of pre-ribosomal RNA and pre-assembly of small nucleolar ribonucleoprotein (snoRNP) complexes on nascent ribosomal RNA, thereby driving early steps of ribosome biogenesis; it contains an N-terminal glycine- and arginine-rich (GAR) domain that mediates nucleolar localization and interaction with box C/D snoRNAs, followed by a central RNA-binding domain and a C-terminal S-adenosyl-L-methionine-dependent methyltransferase motif, localizes predominantly to the dense fibrillar component of the nucleolus in an Nop56/Nop58- and 15.5 kDa protein-dependent manner, and is recruited to sites of rDNA transcription by UBF and SL1; mutations or depletion of fibrillarin impair rRNA processing, leading to defects in 18S and 28S rRNA maturation, nucleolar stress, cell-cycle arrest, and embryonic lethality in model organisms, while in humans its overexpression is linked to several cancers and its absence causes the neurodevelopmental disorder Néstor-Guillermo progeria syndrome, underscoring its dual role as a guardian of ribosome production and a sensor of nucleolar integrity.
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