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Description
Rat DEFB2 (Defensin Beta 2) ELISA KitThe Rat (DEFB2) Defensin Beta 2 ELISA Kit measures Defensin Beta 2 in Rat samples. The plate has been pre coated with Rat DEFB2 antibody. DEFB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat DEFB2 Antibody is added and binds to DEFB2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated DEFB2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Rat (DEFB2) Defensin Beta 2 ELISA Kit measures Defensin Beta 2 in Rat samples. The plate has been pre-coated with Rat DEFB2 antibody. DEFB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat DEFB2 Antibody is added and binds to DEFB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DEFB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat DEFB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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